SrasV1, Main, Exploration, bibRecord, 005C60

[Expression and purification of recombinant SARS coronavirus spike protein].

Identifieur interne : 005C60 ( Main/Exploration ); précédent : 005C59; suivant : 005C61

[Expression and purification of recombinant SARS coronavirus spike protein].

Auteurs : Hao Yu [République populaire de Chine] ; Yong Yang ; Wei Zhang ; You-Hua Xie ; Jun Qin ; Yuan Wang ; Hua-Bao Zheng ; Guo-Ping Zhao ; Sheng Yang ; Wei-Hong Jiang

Source :

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica [ 0582-9879 ] ; 2003.

RBID : pubmed:12897976

Descripteurs français

English descriptors

KwdEn :
- Blotting, Western, Escherichia coli (genetics), Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (immunology), Membrane Glycoproteins (isolation & purification), Recombinant Fusion Proteins (biosynthesis), Recombinant Fusion Proteins (immunology), Recombinant Fusion Proteins (isolation & purification), SARS Virus (chemistry), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (immunology), Viral Envelope Proteins (isolation & purification).
MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Recombinant Fusion Proteins, Viral Envelope Proteins.
- chemistry : SARS Virus.
- genetics : Escherichia coli.
- chemical , immunology : Membrane Glycoproteins, Recombinant Fusion Proteins, SARS Virus, Viral Envelope Proteins.
- chemical , isolation & purification : Membrane Glycoproteins, Recombinant Fusion Proteins, Viral Envelope Proteins.
- Blotting, Western, Spike Glycoprotein, Coronavirus.

Abstract

A novel coronavirus (SARS-coronavirus, SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS) recently. The first step in coronavirus infection is binding of the viral spike protein to certain receptor on host cells. The spike protein is the main surface antigen of the coronavirus and there should be antibodies against spike protein in patients serum. Thus, to develop and expression protein fragment from spike protein gene are the purposes of this experiment. Partial spike gene fragments (751-1925 bp, 2005-3410 bp, 1-1925 bp and 32-3659 bp) and its intact gene were cloned into pET32 or pGEX vectors, and transformed into competent Escherichia coli BL21(DE3) (pLysS), respectively. 63, 78, 98, 160 and 164 kD fusion proteins were successfully expressed with amounts of 35%, 34%, 24%, 17% and 5% of total cell protein. The soluble parts of the cell crude extract were then partially purified by GST affinity chromatography.

PubMed: 12897976

Affiliations:

République populaire de Chine

Le document en format XML

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<author><name sortKey="Xie, You Hua" sort="Xie, You Hua" uniqKey="Xie Y" first="You-Hua" last="Xie">You-Hua Xie</name>
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<author><name sortKey="Qin, Jun" sort="Qin, Jun" uniqKey="Qin J" first="Jun" last="Qin">Jun Qin</name>
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<author><name sortKey="Wang, Yuan" sort="Wang, Yuan" uniqKey="Wang Y" first="Yuan" last="Wang">Yuan Wang</name>
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<author><name sortKey="Zheng, Hua Bao" sort="Zheng, Hua Bao" uniqKey="Zheng H" first="Hua-Bao" last="Zheng">Hua-Bao Zheng</name>
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<author><name sortKey="Zhao, Guo Ping" sort="Zhao, Guo Ping" uniqKey="Zhao G" first="Guo-Ping" last="Zhao">Guo-Ping Zhao</name>
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<author><name sortKey="Yang, Sheng" sort="Yang, Sheng" uniqKey="Yang S" first="Sheng" last="Yang">Sheng Yang</name>
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<author><name sortKey="Wang, Yuan" sort="Wang, Yuan" uniqKey="Wang Y" first="Yuan" last="Wang">Yuan Wang</name>
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<author><name sortKey="Zheng, Hua Bao" sort="Zheng, Hua Bao" uniqKey="Zheng H" first="Hua-Bao" last="Zheng">Hua-Bao Zheng</name>
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<author><name sortKey="Zhao, Guo Ping" sort="Zhao, Guo Ping" uniqKey="Zhao G" first="Guo-Ping" last="Zhao">Guo-Ping Zhao</name>
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<term>Membrane Glycoproteins (immunology)</term>
<term>Membrane Glycoproteins (isolation & purification)</term>
<term>Recombinant Fusion Proteins (biosynthesis)</term>
<term>Recombinant Fusion Proteins (immunology)</term>
<term>Recombinant Fusion Proteins (isolation & purification)</term>
<term>SARS Virus (chemistry)</term>
<term>SARS Virus (immunology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
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<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Envelope Proteins (isolation & purification)</term>
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<term>Glycoprotéines membranaires (immunologie)</term>
<term>Glycoprotéines membranaires (isolement et purification)</term>
<term>Protéines de fusion recombinantes (biosynthèse)</term>
<term>Protéines de fusion recombinantes (immunologie)</term>
<term>Protéines de fusion recombinantes (isolement et purification)</term>
<term>Protéines de l'enveloppe virale (biosynthèse)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines de l'enveloppe virale (isolement et purification)</term>
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<term>Virus du SRAS (immunologie)</term>
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<term>Protéines de fusion recombinantes</term>
<term>Protéines de l'enveloppe virale</term>
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<front><div type="abstract" xml:lang="en">A novel coronavirus (SARS-coronavirus, SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS) recently. The first step in coronavirus infection is binding of the viral spike protein to certain receptor on host cells. The spike protein is the main surface antigen of the coronavirus and there should be antibodies against spike protein in patients serum. Thus, to develop and expression protein fragment from spike protein gene are the purposes of this experiment. Partial spike gene fragments (751-1925 bp, 2005-3410 bp, 1-1925 bp and 32-3659 bp) and its intact gene were cloned into pET32 or pGEX vectors, and transformed into competent Escherichia coli BL21(DE3) (pLysS), respectively. 63, 78, 98, 160 and 164 kD fusion proteins were successfully expressed with amounts of 35%, 34%, 24%, 17% and 5% of total cell protein. The soluble parts of the cell crude extract were then partially purified by GST affinity chromatography.</div>
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<tree><noCountry><name sortKey="Jiang, Wei Hong" sort="Jiang, Wei Hong" uniqKey="Jiang W" first="Wei-Hong" last="Jiang">Wei-Hong Jiang</name>
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[Expression and purification of recombinant SARS coronavirus spike protein].

[Expression and purification of recombinant SARS coronavirus spike protein].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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